A study of sensory and motor Schwann cell culture in vitro
Abstract：Aim To establish the culture method of sensory and motor Schwann cells for the use of nerve tissue engineering and study their biological differences.Methods Sensory and motor Schwann cells were cultured respectively from dorsal root nerves and femoral nerve's motor rami of 5- day old SD rat.The cultured Schwann cells were identified by anti- S100 immuno- fluorescent immunohistochemical staining,and the NGF expressions of two kinds of cells in the culture media were measured by Sandwich ELISA. Results Both kinds of cells were proved to be Schwann cells.No obvious difference was observed at morphology under light microscope, but their NGF expressions were different in both quantity and pattern (F=45.3681, P=0.000).Conclusion High purity of sensory and motor Schwann cells needed by nerve tissue engineering can be prepared by this method, and there is biological difference between them.
Keywords：Schwann celltissue engineeringnerve regenerationnerve growth factor
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2302-2303 )