Effects of hyperbaric oxygen on the expression of Bcl- 2 and Bax proteins in CA1 area of gerbil hippocampus after forebrain ischemia and reperfusion
Abstract：Aim To observe the expressions of Bcl- 2 and Bax proteins at CA1 area of hippocampus in hyperbaric oxygen(HBO) and explore the mechanisms of hyperbaric oxygen treatment ( HBOT ) reducing neuronal apoptosis following forebrain ischemia reperfusion.Methods 20 gerbils were randomly divided by random number table into normal control group,ischemia group,0.15 MPa HBO, 0.25 MPa HBO and 0.25 MPa hypervaric air (HBA) control group with 4 gerbils in each group.Forebrain ischemia model was established by bilateral common carotid arteries blocking.Animals were reperfused for 3 days after 20 minutes of ischemia and treated by 0.15 MPa and 0.25 MPa of hyperbaric oxygen(60 min/d for 3 days).Thereafter,expressions of Bcl- 2 and Bax protein were observed by immunohistochemical labelled streptavidin biotin (LSAB) method.Results The expression of protein Bcl- 2 in hippocampus CA1 was significantly increased in HBOT groups (P< 0.01), and changes in 0.25 MPa groups were greater than those in 0.15 MPa groups (P< 0.01).The expression of protein Bax in hippocampus CA1 was not changed significantly in HBOT groups.Conclusion HBO can induce the expression of Bcl- 2,which is the mechanism of neuronal protecting effect of HBOT.
Keywords：hyperbaric oxygenationreperfusiongenesBcl- 2
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2278-2279 )