Differentiation of mouse embryonic stem cells after transplantation into the adult rat brain
Abstract：Aim To observe differentiation of ES cells of mice in adult rat brain after transplantation into septum and hippocampus.Methods 35 adult SD rats weighed 200- 250 g without limitation of sex were used.ES- BALB/C cells were implanted into hippocampus and septum of adult rat,brain was taken at 1st,2nd,3th,4th,8th week.Sections were frozen and Nissl's staining,M6,NSE,GFAP immunohistochemical staining were performed.Results ES grafts expressed M6,GFAP,NSE antigen from second week after implantation into septum and hippocampus which were mainly located in implantation region.Conclusion Transplanted mice ES cells can differentiate into neurons, glia in the septum and hippocampus of rat brain.
Keywords：brain tissue transplantationcell differentiationimmunohistochemistry
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2258-2259 )