Detection of tissue inhibitors of metalloproteinases in rat cardiac fibroblasts
Abstract：Aim To investigate the mRNA levels of tissue inhibitor of metalloproteinase-1(TIMP-1),tissue inhibitor of metalloproteinase-2 (TIMP-2) in rat cardiac fibroblasts by multiplex reverse transcription-polymerase chain reaction (RT-PCR). Methods Cardiac fibroblasts of adult female Sprague-Dawley rats were isolated. Total RNA was extracted and then reverse transcription was performed. Multiplex RT-PCR was carried out and PCR products were analyzed by agarose gel electrophoresis. Results 2％ agarose gel electrophoresis showed that there were three targeted PCR products in a single lane which matched rat TIMP-1, TIMP-2 and GAPDH respectively. Conclusion We establish a feasible, simple and sensitive approach to measure mRNA of rat TIMP-1, TIMP-2 by multiplex RT-PCR, which may contribute to the research on cardiac fibrosis associated with hypertension.
Keywords：fibroblastrattissue inhibitor of metalloproteinasereverse transcriptase polymerase chain reaction
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2154-2155 )