Expression of Smad3 mRNA and Smad7 mRNA of TGF β 1 in keloid-derived fibroblasts
Abstract：Aim To study the expression of Smad3 mRNA and Smad7 mRNA of transforming growth factor β 1 ( TGF β 1) on keloid-derived fibroblasts. Methods The expression of Smad3 mRNA(at 1,2,4,24,48 h)and Smad7 mRNA(at 0.5 1,1.5, 2,4,24 h) were detected by using RT-PCR after TGF β 1 in different concentration(0,5,50,500 pmol/L) were added to the monolayer culture system. Results Expression of Smad3 mRNA decreased with increase of TGF β 1 in a concentration dependent manner.Contrary to Smad3,5 pmol/L TGF β 1 significatnly enhanced the expression of Smad7 by 2.6 time( 46± 7,control group 13± 7,t=6.150, P=0.0005) ,its expression increased with increase of TGF β 1 dose indicating Smad7 mRNA was very sensitive to TGF β 1 stimulation. Smad3 mRNA level decreased after TGF β 1 stimulation and reached the minimum value 48 hours later ( 53± 9,control group 18± 8,t=6.011, P=0.0005) and showed time dependence; Smad7 mRNA level was increased compared with control group ( 73± 11,control group 19± 10,t=5.215, P=0.003) ,24 h later,returned to the level similar to that in control group.Conclusion TGF β 1 may down regulate the expression of Smad3 mRNA,but up regulate that of Smad7 mRNA in a ligand-dependent way.
Keywords：transforming growth factor betakeloidfibroblasts
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2004-2005 )