Effect of FADD-DN transfection on He-Ne laser-inducing apoptosis of scar fibroblasts in vitro
Abstract：Aim To explore the molecular mechanism of He-Ne laser-inducing apoptosis of scar fibroblasts in vitro.Methods These fibroblasts-devired from hypertrophic scar (HS) were cultured and were divided into three groups:transfection group (TG),control transfection group (CTG) and non-transfection group (NTG).The fibroblasts in TG were co- transfected with GFP (green fluorescent protein) cDNA and pcDNA3-FADD-DN. Scar fibroblasts in CTG were cotransfected with GFP cDNA and pcDNA3. The fibroblasts in NTG was't transfected. Scar fibroblasts of each group were irradiated with He-Ne laser (100 mW/cm2 for 30 minutes) at 24 hours after transfection, once a day for 3 consecutive days.Apoptosis of cell was then examined by TUNEL technique. Results The percent of apoptosis in TG was 9.27% and that of apoptosis was 15.73% in CTG, and 12.87% in NTG.The percent of apoptosis in TG was less than in CTG (q=4.55,P< 0.01) and NTG(q=4.14,P< 0.05) respectively.Conclusion FADD-DN gene transfection can depress He-Ne laser-inducing apoptosis of scar fibroblasts.FADD may be an essential component of the He-Ne laser-activited cell death pathway.
Keywords：laserapoptosisfibroblastsgenetransfection
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2002-2003 )