Cloning cDNA of the recombinant IN-1 single-chain antibody and its role in regeneration of central nerves
Abstract：Aim IgMG type of IN-1 gene was modify and cDNA segments of IN-1 recombinant single chain antibody was developed by gene engineering which is a new way for regeneration promotion of central nerves and treatment of diffused axonal injury.Methods Host bacterium was E. Coli DH5a,and cloning plasmid was pUC18.Referring to light and heavy chain published in GeneBank,we redesigned gene segments which were suitable to express in E. Coli.35 segments with the length ranging from 40- 50 bp were assembled in only one step by a PCR approach.The entire gene was cloned into cloning plasmid vector pUC18.The sequence of the cloned cDNA was confirmed by DNA sequncing, clone PCR and restriction enzymes analysis.Results Sequence analysis showed that the splicing order, the direction and the sequence in the gene were almost correct except for one nucleotide acid.Conclusion The successful construction of the recombinant vector pUC18 bearing the cDNA might provide materials for the further research on the IN-1 single-chain antibody.
Keywords：genetic vectorsgenessynthetic
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 1904-1905 )