Immunohistochemical study of hyalinosis of microvessels in the human brain
Abstract：Aim Immumohistochemical method was used to study 8 patients with hyalinosis of microvessels,to explore pathogenic mechanism of hyalinosis of microvessels in the human brain.Methods Autopsy material was obtained from 8 patients with morphological signs of hyalinosis of intracerebral microvessels identified with haematoxylin eosin stain.Eight cases(5 males,3 females,28- 80 years of age) without signs of brain pathology were used as controls;four of them died of heart diseases and four from malignant tumours.These cases have been used as controls in two parallel studies: Binswanger' encephalopathy and congophilic angiopathy.Immumohistochemical ABC method with collagens type I,II,IV,VI,actin,ulex,factor VIII,CD-34,glucose transporter 1 antidoby were used to study 8 cases with hyalinosis of microvessels and 8 cases without encephalopathy.Results collagens type I,II,IV,VI were stacked at middle layer and external layer of microvessels vascular wall abnormally.Basal layer components,collagen type IV and laminin mostly stacked at basement membrane of blood vessel and smooth muscle layer of middle layer.Conclusion Degeneration of smooth muscle cells of the media and deposition of collagens and other substances probably reduce the capacity of hyaline arterioles to respond to neurogenic and metabolic influences, thereby jeopardizing their ability to modulate cerebral blood flow.
Keywords：cerebrovascular accidentcollagenimmunohistochemistryvascular disease
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1886-1887 )