Effect of 17β- estradiol on the gene expression of core- binding factor α 1 and osteoblastogenesis in bone marrow stromal cells
Abstract：Aim To investigate the effect of 17β- estradiol (E2) on the gene expression of core binding factor alpha 1(Cbfα 1) during the differentiation of rat bone marrow stromal cells exposured to the differentiation medium and to elucidate the effects of E2 on osteoblastogenesis.Methods Adherent bone marrow stromal cells from 3- month- old female SD rat were treated with DEX 1× 10－ 7 mol/L , 1,25(OH)2D3 1× 10－ 9mol/L and different concentrations (0－ 1× 10－ 6mol/L)of E2.Total cellular RNA was isolated using total RNA kit.The effect of E2 on the expression of Cbfα 1 mRNA was quantified by semiquantitative RT- PCR based on the comparison with an internal reference, β- actin expression, and demonstrated by Northern blot.Results E2 strongly inhibited the expression of Cbfα 1 mRNA in bone marrow stromal cells during differentiation from 23.4％± 1.8％ to 15.8％± 1.5％ （ t=6.3,P< 0.01） and 5.8％± 0.8％（ t=17.8,P< 0.05） under different concentrations (0,1× 10－ 10, 1× 10－ 8,1× 10－ 6mol/L ) of E2.The suppression of the Cbfα 1 mRNA was dose dependent.Northern blot showed the expression of Cbfα 1 mRNA were decreased from 4.22± 0.11 to 2.51± 0.12(t=21, P< 0.01),1.88± 0.10(t=31, P< 0.01) and 1.17± 0.09( t=41,P< 0.01).The activity of ALP decreased significantly at higher concentrations of E2 from (42.6± 2.5) U/g(protein) to （ 17.9± 2.0） U/g(protein) ( t=15,P< 0.01),(10.8± 1.5) U/g(protein) ( t=21,P< 0.01) and (3.6± 0.7)U/g(protein)（ t=29,P< 0.01） .The amount of type Ⅰ collagen was suppressed by E2.Conclusion E2 can suppress Cbfα 1 gene expression in bone marrow stromal cells in differentiation medium and inhibit osteoblastogenesis.
Keywords：estradiol/pharmacologybone marrow stromal cell/cytologycell culture
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1626-1628 )