The effect of radiation injury on histiocyte of skin wound and the repaired- promoting action of Kangfuxin

doi:10.3321/j.issn:1673-8225.2003.11.010

Abstract：Aim To investigate the effect of radiation injury of whole body on histiocyte in skin wound and Kangfuxin(W11-α 12) and explore the mechanism of difficult recovery of combined wound and measures.Methods 100 male and healthy mice of Kunming series weighted (20± 2) g.The experimental group of simple wound and radiation damaged combined with trauma was made respectively.The rats irradiation was accomplished with a dose of 6 Gy using a 60Co γ- ray irradiation,then two incisions wound on back were made.To observe speed of wound healing and content changes inflammatory cells in wound,fibroblast,quantity of blood capillary and bFGF.Results On the 3rd,5th and 7th day after injury,the recovery speed(% ) of wound in simple wound group was 63.22% ,83.47% and 94.61% respectively and that in the combined wound group obviously decreased(t=7.84,7.26,8.18,P< 0.01) and was 52.67% ,69.18% and 82.88% respectively.On the 1st,3rd and 5th day after burn,the quantity of inflammatory cells in the combined wound group decreased 26.53% ,62.11% and 38.91% respectively compared with the control group,that of fibroblast decreased 43.73% ,52.70% and 25.32% and that of blood capillary decreased 55.97% 5 days after injury.After using Kangfuxin,step- up of wound healing and increasing of cell quantity could be seen in both groups,but it was better in the combined group than the control group.On the 3rd and 5th day after injury,the bFGF content of epidermic cell in the combined group was lower 59.15% and 36.15% compared with the simple wound group.After using Kangfuxin,the width of increasing in the combined wound group increased 35.90% and 10.97% respectively than the single wound group.Conclusion Decreasing of local cell quantity in wound after injury and reducing of bFGF content in epidemic cells is one of the important factors causing the difficult recovery of wound surface.Kangfuxin has improving effect on the migration and proliferation of cells and the function of epidemic cell's excreting bFGF.