Effects of glial derived neurotrophic factor on focal cerebral ischmia in rats
Abstract：Aim By studying the influence of glial derived neurotrophic factor(GDNF) on the focal cerebral ischemia,the expression of Caspase - 3 and neuronal apoptosis,to investigate the role of GDNF in cerebral ischemia and its possible mechanism.Methods 120 healthy male Wistar rats were divided randomly into GDNF group and saline group.Each group were divided further into shamed operation subgroup and 0,3,6 and 24 h subgroup according to the time exposed to ischemia.The model of middle cerebral artery occlusion was performed with intraluminal filament occlusion.At the same time of occlusion,5 μ l of GDNF and saline was injected respectively into the cerebral ventricle of the rats.The infarction volume percent,expression of Caspase - 3 and neuronal apoptosis were observed.Results The cerebral infarction volume of GDNF group was significantly smaller than those of saline group.Neuron injury of GDNF group was less serious than saline group,and it's noteworthy that hippocampus neuron even bore no obvious injury.A significant decrease of the number of Caspase - 3 and TUNEL staining positive neuron was detected also in GDNF group.Conclusion GDNF does have protective effects on focal cerebral ischemia. Inhibiting the expression of Caspase - 3 and neuronal apoptosis may be one of the possible mechanisms of the event.
Keywords：brain ischemianerve growth factorscysteine endopeptidases
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 1504-1505 )