Expression of inhibiting protein of cell cycle-p27kip1 and cyclinE proteins and their relationships with the genesis and prognosis of human colorectal adenocarcinoma

doi:10.3321/j.issn:1673-8225.2003.08.009

Abstract：Aim The relationships between p27kip1 protein as negative factor of cell cycle regulation and colorectal adenocarcinoma have been seldomly reported in China.This article aims to investigate the effects of inhibiting factor of cell cycle regulation-p27kip1 and cyclinE proteins in the genesis and progression of human colorectal adenocarcinoma.Methods The expressions of p27kip1 and cyclinE proteins in tumor tissues of 37 patients with colorectal adenocarcinoma and in normal colorectal mucosal tissues of 26 subjects were detected by SP immunohistochemical technique.Results p27kip1 protein positive-expression rate in tumor tissues of colorectal adenocarcinoma was 45.9％ , which was lower than that in normal colorectal mucosal tissues (χ 2=4.590,P< 0.05)， p27kip1 protein positive-expression correlated significantly with tumor cell differentiation and lymph node metastasis (χ 2=9.653;χ 2=6.838,P< 0.05)； cyclinE positive-expression rate in tumor tissues was 64.9％， which was higher than that in normal colorectal mucosal tissues (χ 2=4.285,P< 0.05)； cyclinE positive-expression also correlated significantly with tumor cell differentiation and lymph node metastasis (χ 2=8.397;χ 2=5.261,P< 0.05); cyclinE protein positive-expression rate in the tumor tissues of p27kip1 protein positive-expression group was lower than that in p27kip1 protein negative-expression group, there were no significantly correlation between the two groups ( r =－ 0.1133, P >0.05).Conclusion The decreased expression of p27kip1 protein and/or over－ expression of cyclinE protein may play an important role in the genesis of colorectal adenocarcinoma and might be helpful to the determination of malignant degree and lymph node metastasis as well as the treatment and living quality of the diseases.