Metabolic Engineering Modifications of the L-leucine Producing Strain Corynebacterium glutamicum and Its Fermentation Efficiency
HUANG Qin-geng
Abstract：Taking Corynebacteriumglutamicum MD106 as a starting strain applied to produce L-leucine,the mutant strain MD106ΔpanBC/ΔalaT from the double gene-negative of panBC and alaT was structured by using the meth-ods of overlap extension PCR and suicide vector-mediated homologous recombination,and the fermentation index was measured through the ermentation test of the starting strain and the recombinant strain. The results showed that the L-leucine yield of the recombinant strain was 7.91g/L by Amino acid analyzer after40 h fermentation,and it increasing by 43.3% comparing with that of the original strain MD106,and mainly miscellaneous acid---alanine reduced by more than 80%,the total ration of miscellaneous acid decreased 55.6% compared with the original strain.
Keywords：L-leucineCorynebacterium glutamicummetabolic engineeringmiscellaneous acid
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 68-72 )