Effects of drug-containing serum of Liuwei Dihuang Pill on Wnt/β-catenin pathway,migration and invasion of trophoblast cells induced by high glucose

ZHU Juhua; CHEN Kang; JIN Ye

doi:10.11958/20251508

Abstract：Objective To investigate the effect of drug-containing serum from Liuwei Dihuang Pill on Wnt/β-catenin pathway,migration and invasion of trophoblast cells induced by high glucose.Methods The drug-containing serum of Liuwei Dihuang Pill was prepared.Human chorionic trophoblast HTR-8/Svneo cells were cultured in vitro and divided into the control group(5 mmol/L glucose+blank serum),the high glucose group(30 mmol/L glucose+blank serum),the low-dose group(30 mmol/L glucose,serum medicine solution of Rehmannia Six Formula at 0.45 g/mL),the medium-dose group(30 mmol/L glucose,serum medicine solution of Rehmannia Six Formula at 0.90 g/mL)and the high-dose group(30 mmol/L glucose,serum medicine solution of Rehmannia Six Formula at 1.35 g/mL).The mRNA expressions of Wnt1,β-catenin and Cyclin D1 in HTR-8/Svneo cells were detected by real-time fluorescence quantitative PCR(qRT-PCR).The protein expressions of Wnt1,β-catenin,phosphorylated glycogen synthesis kinase-3β(p-GSK-3β)and Cyclin D1 in HTR-8/Svneo cells were detected by Western blot assay.The migration and invasion of HTR-8/Svneo cells were detected by Transwell method.Results Compared with the control group,the protein and mRNA expression levels of Wnt1,β-catenin and Cyclin D1,the numbers of migration cells and invasive cells in HTR-8/Svneo cells were decreased in the high glucose group(P<0.05).Compared with the high glucose group,the protein and mRNA expression levels of Wnt1,β-catenin and Cyclin D1,the numbers of migration cells and invasive cells were significantly increased in the low dose,the medium dose and the high dose groups of the HTR-8/Svneo cells(P<0.05).In addition,with the increase of the drug containing serum dose of Liuwei Dihuang Pill,the above indexes in HTR-8/Svneo cells increased successively(P<0.05).Conclusion The drug containing serum of Liuwei Dihuang Pil can activate Wnt/β-catenin pathway in HTR-8/Svneo cells induced by high glucose,and promote the migration and invasion of HTR-8/Svneo cells.