Impact of sufentanil on liver injury in burn-induced sepsis rats by regulating JAK2/STAT3 signaling pathway

XU Fang; LIANG Yi; HE Yong; XU Julong

doi:10.11958/20240681

Abstract：Objective To investigate the effect of sufentanil(suf)on hepatic injury in burn sepsis rats by regulating Janus kinase 2(JAK2)/signal transduction and transcriptional activator 3(STAT3)signaling pathway.Methods SD rats were randomly divided into the control group,the model group,the suf low-dose group(0.75 µg/kg),the suf high-dose group(3 µg/kg),the imipenem group(90 mg/kg)and the suf high-dose+kumamycin group(3 µg/kg suf+4 mg/kg kumamycin).Serum liver function indexes including alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were detected.The pathological changes of liver tissue were observed by HE staining.The contents of tumor necrosis factor-α(TNF-α),monocyte chemoattractant protein-1(MCP-1)and interleukin(IL)-6 were detected by enzyme-linked immunosorbent assay(ELISA).Terminal deoxynucleotide transferase mediated dUTP nick end labeling(TUNEL)staining was used to detect hepatocyte apoptosis.Western blot assay was used to detect the expression of p-JAK2 and p-STAT3 proteins in liver tissue.Results Compared with the control group,the model group showed elevated serum levels of ALT and AST,swelling of liver cells and infiltration of numerous inflammatory cells in liver tissue.The levels of TNF-α,MCP-1 and IL-6 in liver tissue,apoptosis rate of liver cells,and expression levels of p-JAK2 and p-STAT3 proteins were increased(P＜0.05).Compared with the model group,serum levels of ALT and AST were reduced in the suf low-dose group and the suf high-dose group,and pathological damages of liver tissue were improved.The contents of TNF-α,MCP-1 and IL-6 in liver tissue,the apoptosis rate of liver cells,and the expression levels of p-JAK2 and p-STAT3 proteins were reduced(P＜0.05).Moreover,changes in above indicators were significantly higher in the suf high dose group of suf than those in the suf low dose group(P＜0.05),and there was no significant difference compared with the imipenem group.Kumamycin inhibited the improvement effect of high-dose suf on liver injury in rats with burn sepsis(P＞0.05).Conclusion Sufentanil may improve liver injury in burn-induced sepsis rats by inhibiting the JAK2/STAT3 signaling pathway.