DOI: 10.11958/20170998
Antitumor effects of DC-GPC3 cocultured with CIK on hepatocellular carcinoma in vitro
HAN Qiu-qing
JIANG Jin
WANG Yu-liang
Abstract：Objective To investigate biological activity and antitumor effects of phosphatidylinositol-3 (GPC3) gene transfected dendritic cells(DC,DC-GPC3)co-cultured with cytokine induced killer cells(CIK)on hepatocellular carcinoma (HCC). Methods The phenotypes of effector cells were analyzed by flow cytometry. Levels of interleukin (IL)-2 and interferon (IFN)–γ were determined by MTT colorimetry and enzyme-linked immunosorbent assay, respectively. The cytotoxic activity against hepatocarcinoma cells (HepG2) was measured by lactate dehydrogenase release. Results The proportions of CD3+CD8+and CD3+CD56+double-positive cells were significantly elevated in the DCIK-GPC3 compared with the DCIK and CIK(P<0.05).Compared with the DCIK and CIK,the DCIK-GPC3 showed significantly higher levels of secreted IL-2 and IFN-γ in the supernatants(P<0.05).The antitumor effect of DCIK-GPC3 against HepG2 was the highest than that of DCIK and CIK at an effector-target ratio ranging from 20:1 to 50:1(P<0.05).Conclusion DCIK-GPC3 can enhance the cytotoxic activity against hepatocarcinoma cells in vitro.This study provides a theoretical and experimental basis for clinical immunotherapy using DCIK-GPC3.
Keywords：glypicandendritic cellscytokinesliver neoplasmsinterleukin-2
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 118-121 )