DOI: 10.11958/20160444
Expression and identification of VEGF165 in bone marrow mesenchymal stem cells of rhesus
GUO Zaiyu
ZHANG Heliang
SHUI Tao
ZHANG Guozhe
ZHAO Weihua
CHEN Qian
HOU Yanwei
Abstract：Objective To detect the transferred vascular endothelial growth factor (VEGF)165 gene expression in rhesus autologous bone marrow mesenchymal stem cells (MSCs), and to explore the functional viability of transgenic MSCs. Methods MSCs from rhesus bone were isolated by Ficoll, which were used to detect the phenotype. After the culturing, the expression vector pcDNA-eGFP-VEGF165 was transfected into bone marrow MSCs. Fluorescence microscope and flow cytometry were used to detect the enhanced green fluorescent protein (eGFP) expression. At the same time, the phenotype in transfected MSCs was also indentified. The VEGF165 expression level was detected by RT-PCR. Results The highly purified MSCs were collected successfully. The transfected MSCs and daughter cells showed expressions of eGFP and VEGF165, which also remained the characteristics of MSCs. Conclusion The VEGF165 gene that is transfected into MSCs can maintain characteristics of MSCs, and stably express foreign genes.
Keywords：mesenchymal stem cellsvascular endothelial growth factormacaca mulattatransgenesmesenchymal stem cell transplantationgreen fluorescent proteins
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1209-1212 )