The effects of KS tablet on hematopoietic regulation in mice treated with radiation

doi:10.11958/20150143

Abstract：Objective To investigate the effects of Kangshuai (KS) tablet on the hematopoietic reconstitution in mice treated with radiation. Methods C57BL/6 mice were randomly divided into four groups:irradiated control group, low-dose group (0.75 g/kg), middle-dose group (1.5 g/kg) and high-dose group (3.0 g/kg). There were ten mice for each group. The mice in three treated groups were given KS tablet suspension orally for three days before the treatment with irradiation, and the control group was given equal quantity of normal saline. All the mice were underwent irradiation of 6.0 Gy 137Cs-γrays, and were continuously given drug suspension or saline for seven days. The mice were sacrificed on the eighth day after irradiation. Colony forming unit of spleen (CSF-S), granulocyte macrophage colony-forming unit (CFU-GM) and fibroblast colony-form-ing unit (CFU-F). The 2D CFU-GM hematopoietic stem cells (HSC) were co-cultured with mesenchymal stem cell (MSC). Re-sults Compared with the irradiated control group, numbers of CFU-GM were significantly increased in three treatment groups. The proliferations of CFU-S, CFU-F and 2D CFU-GM were significantly enhanced in middle and high dose groups (P<0.05). Conclusion KS tablet can promote hematopoietic reconstitution in mice after irradiation damage.