Effects of down-regulatedβ-catenin on epithelial mesenchymal transition, invasion and migration ability of breast cancer cell line MDA-MB-231
Abstract：Objective To investigate the effect ofβ-catenin on epithelial mesenchymal phenotype and invasion migra?tion ability of breast cancer cell line MDA-MB-231. Methods Small interfering RNA (siRNA) targetingβ-catenin plas?mid was transfected into MDA-MB-231 cell line. Immunofluorescence staining was used to observe the expression of β-catenin. The expressions of epithelial cell marker E-cadherin and mesenchymal marker vimentin, epithelial mesenchymal transition correlation factor Twist1 and Snail were detected by Western blot assay. Invasion and migration ability was com?pared by transwell invasion and wound healing assay between control group, the MDA-MB-231 group andβ-catenin down-regulated group. Results Immunofluorescence staining showed thatβ-catenin was expressed in cell membrane, cytoplasm or nucleus in MDA-MB-231 group and control group. There was a decreased expression in β-catenin down-regulated group, and no expression in cytoplasm or nucleus. The expression of E-cadherin was increased, while vimentin, Twist 1 and Snail expression decreased inβ-catenin down-regulated cells. Transwell invasion and wound healing assay results proved that transmembrane cell number and migration distance were significantly lower in β-catenin down-regulated group than those of MDA-MB-231 group and control group (P<0.05). Conclusion The down-regulation ofβ-catenin inhibits Wnt/β-catenin activation that decreases the mesenchymal phenotype but increases epithelial phenotype of breast cancer cells MDA-MB-231, and which reduces the cell invasion and migration ability in vitro.
Keywords：breast neoplasmscadherinsneoplasm invasivenesscell movementβ-cateninepithelial mesenchymal transitionMDA-MB-231
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 981-984,1093 )