The Expression of EGFL-7 in Brain Tissue of Type 2 Diabetic Rats and the Adjusting Function of Amioguanidine and Baicalein

WANG Qiong; LI Huimian

doi:10.3969/j.issn.0253-9896.2014.11.003

Abstract：Objective To investigate the expression of epidermal growth factor-like domain 7 (EGFL-7) in brain tis?sue of diabetes mellitus (DM) rats, and the intervene effect of amioguanidine and baicalein thereof. Methods Forty-eight SD rats were randomly divided into following four groups:control group, diabetic group, aminoguanidine group and baicalein group (n=12 for each group). The control group received only pelleted food and water. Diabetic group, aminoguanidine group and baicalein group were given high fat and sugar diet in combination with small dose of intraperitoneal injection of strepto?zotocin to establish the diabetic model. And then aminoguanidine group and baicalein group were given aminoguanidine and baicalein 150 mg/(kg · d) through oral gavaging. Diabetic group and control group were given equivalent saline through oral gavaging. After 16-week treatment, the expression of EGFL-7 proteins in rat brain was detected by immunohistochemistry and Western blotting methods. Results Results of immunohistochemistry and Western blotting assay showed that the ex?pression of EGFL-7 was significantly increased in diabetic group, aminoguanidine group and baicalein group compared with that of control group (P<0.01). When compared with diabetic group, the expression of EGFL-7 was significantly decreased in aminoguanidine group and baicalein group (P<0.01). There was no significant difference in the EGFL-7 expression be?tween aminoguanidine group and baicalein group (P>0.05). Conclusion EGFL-7 may play an important role in cerebral vascular hyperplasia and remodeling of diabetic rats. Aminoguanidine and baicalein restrained cerebral vessel growth by re?ducing the expression of EGFL-7.