Isolation and Culture of Epidermal Stem Cells from the Third Generation of Mice That Were Knocked-out of Terc
ZHANG Junling
HUANG Song
LU Lu
LI Deguan
WU Hongying
MENG Aimin
Abstract：Objective To observe the difference in number of epidermal stem cell and its function between wild-type (WT) mice and the third generation of Terc knockout (G3Terc-/-) mice. Methods Flowcytometry was used to analyse and sort epidermal stem cells;Quantitative real-time PCR is used to analyse the relative expression level of p 21 in epider-mal stem cells;Self-renewal ability was reflected by the number of colonies formed by epidermal stem cells. Results Basal and suprabasal ratios in epidermal stem cells in WT mice were (9.56 ± 1.06)% and (1.22 ± 0.08)% respectively; basal and suprabasal ratios in epidermal stem cell in G3Terc-/-mice were (17.36±3.56)%and (2.92±0.72)%respectively. Relative p21 expression level in G3Terc-/-mice was 6.40 fold to WT mice;Number of colonies formed by WT mice epidermal stem cells were (280.20±29.81) per 104 cell, number of colonies formed by G3Terc-/-mice epidermal stem cells were (29.28±5.24) per 104 cell, which present significant difference to each other(P<0.05). Conclusion Compared to WT mice, epidermal stem cells in G3Terc-/-mice were aging.
Keywords：miceknockoutskin agingstem cellstelomerase
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 762-764 )