The Application of DiversiLab System in Klebsiella pneumoniae Genotyping
LI Jing
HU Zhidong
TIAN Bin
XU Hairu
Abstract：Objective To analyze Klebsiella pneumoniae by DiversiLab system, providing scientific evidence for the control of nosocomial infection. Methods Eight strains of non-duplicated clinical Klebsiella pneumoniae isolated from the surgical ward in a hospital in 2010 were typed by rep-PCR-based DiversiLab system. The results were compared with those of pulsed-field gel electrophoresis (PFGE). Results Antimicrobial susceptibility test showed that 7 clinical strains were the same sensitivity to 11 antimicrobial agents, except the strain K8-02. And these 7 clinical strains were all multi-drug re-sistant. The result of PFGE showed that 7 strains were the same pattern. The result of DiversiLab also showed that 7 strains were the same pattern, and the strain K8-02 was another pattern. Conclusion DiversiLab system is simple, quick, good re-peatability and accurate, which is a kind of standardization and automation system. DiversiLab system and PFGE method could get the same result.
Keywords：Klebsiella pneumoniaeelectrophoresisgelpulsed-fieldbacterial typing techniquesDiversiLab system
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1076-1078 )