xperimental Study of Inhibitory Effect of Antisense IGF-I on Glioma Proliferation in Vivo & in Vitro
LI Jie
HUANG Qiang
Pu Peiyu
Abstract：Objective: To study the exact role of IGF-I in the development of glioma and the effective target for gene therapy
against brain tumor. Methods: (1)An antisense IGF-I mRNA strategy was performed in rat C6 glioblastoma cells with lipo-
fectamine assay. The levels of IGF-I expression and cell proliferative potential before and after transfection were compared.
(2) A rodent animal model (Wist ar rat ) beating subcutaneous C6 tumor was set up, through which the different biologic
characters before and after the gene treatment of antisense IGF-I were analyzed. Results: Compared with their parental cells,
the transfected C6 cells that expressed IGF-I on a relative low level cut down their proliferative potential about 35 %. (2)
Within 8 weeks,the tumors treated with antisense IGF-I disappeared completely,while those in control group became large
continuously. Around the tumors,apoptotic cells and mononuclear lymphocytes increased and the IGF-I expression and cell
proliferative potential decreased in the therapeutic group. Conclusion:The antisense IGF-I mRNA strategy might be a sound
therapeutic modality against neoplasms. Its mechanism based not only on the antisense-mediate inhibition of IGF-I expres-
sion but also on the antisense-mediate enhancement of immune response of the host.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 290-292 )