The Quantitative Determination of Serum Amino Acids in Burn Patients by Pre-column Derivatization Reversed-Phase HPLC
Abstract：Objeetive: To quantitatively detemine the content of serum amino acids in patients with severe bum by pre-column de
rivatization reversed-phase high performance liquid chromatography (HPLC). Methods: Using 2,4-dinitrofluorobanzene (2,
4-DNFB) as a derivative agent, the oontents of 17 kinds of free amino acid at 360 nm were detected by reversed-phase
HPLC and calculated by external standard method. Results:The 17 kinds of free amino acid derived from DNFB were com
pletely separated in 27 minutes. Their rates of recovery varied from 97.32% to 104.26% and coefficients of variation were
1.5 %～4.8 %. The obvious changes of content of many amino acids occurred in patients with severe bum. Conclusion: The
reversed-phase HPLC is a simple,rapid and exact method suitable to clinical detection.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 278-280 )