Specific Cellular and Humoral Irmmune Responses Induced by DNA Vaccine of HBV Surface Gene in Mice
Abstract：Objective:To observe the specific immune responses and the protection against P815 mastocytoma cells stable expressing HbsAg in H-2d mice after immunized by DNA vaccine of HBV S gene. Methods: The immunization was performed by intramuscular injection of DNA and subcutaneous injection of P815-HBV-S. Serum anti-HBs was detected by ELISA. HbsAg secific cytotoxic T lymphocyte(CTL) activity was measured by 51 Chromium release assay. Results:The expression of HBsAg was demonstrated by indirect immunofluorescent method. The 450nm A value of serum in immunized mice was 0.87.HBsAg specific CTL activity was 51.1%. HBV-SDNA vaccine could evidently inhibit the tumor growth, prolong the surviv
al period(＞38.2 d), and improve the survival rate. Conclusion: pCR3.1-S DNA vaccine has a strong antigenicity and marked killing effect to HBV infected cells in vivo.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 217-219 )