Effect of Shen Nong 33 on Gene Expression of Tumor Necrosis Factor inLipopolysacchride-Induced Acute Lung Injury Rat
LIU Jiying
LI Jin
WANG Xueqian
Abstract：Objective: To investigate the effect of Shen Nong 33(SN 33)(a traditional Chinese medicine)on gene expression of tu-
mor necrosis factor( TNF-α) in lipopolysacchride(LPS)-induced acute lung injury( ALI)rat. Methods: The levels of TNF-α in
rat serum were measured with ELISA and TNF-α gene expression in rat lung tissue was observed with Northern Blotting at
different time courses. The effects of SN 33 and DXM on release and gene expression of TNF-α and the changes of lung his-
topathology were observed at the same time. Results:The serum levels of TNF-α rapidly increased after LPS injection. TNF-
α gene expression in rat lung tissue was dramatically upregulated during ALI. Both SN 33 and DXM could decreased the se-
rum levels and lung gene expression of TNF-α. Conclusion: TNF-α plays an essential role in an inflammatory response. LPS-
induced ALI may be prevented by SN 33 and DXM.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 165-167 )