Detection of Pap A Gene from Uropathogenic E. coli with Polyrnerase Chain Reaction
Abstract：Objective:To detect pap A gene from uropathologic E. Coli(UPEC) with polymerase chain reaction(PCR) in order to es-tablish an assay to identify UPEC strains. Methods:A pair of oligonucleotide primers derived from the published DNA se-quence of F13 serotype pap A were used in PCR to emplify about 700bp-long pap A gene. As compared with hemagglutina-tion test,PCR method was applied to detect pap A gene of the different serotype P-piliated strains as well as a collection of39 E. Coli strains isolated frorm urine of patients with urinary tract infections. Results: Seven serotype pap A genes were allpositive, while sepecific amplification in negative control strains were not found in PCR detection. The detection rates ofMRHA and papA PCR positive strains inthe tested isolates were 74.4% and 71.8% ,respectively. There was no statisticalsignificance between the above results(P ＞0.05). When bacterial cells were used directly in PCR detection,a sensitivity of 3× 105～106 cfu/ml was demonstrated. Condusion: The pap A PCR method may have a good application prospect in theidentification of UPEC strains.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 160-162 )