Establishment of Radioimmunoassay for Compound W in Human Serum and Urine
Abstract：Objective: To establish a radioimmunoassay(RIA) for compound W in human serum and urine. Methods:Chloramine Tmethod was used in diiodothyromine iodination. After purification, seperation and sulfation, the125 I-T2S was obtained andused in the RIA. The concentrations of compound W in 31 non-pregnant women serum and random spot urine samples weremeasured and the urinary concentration was adjusted for urinary creatinine level. Results:Because of the different agent, thelower limits of detection for the assay in serum and urine were 23.47 fmol/L and 93.55 fmol/L, respectively. The coeffi-cients of variant in the same test of serum and urine were 6.20 % ( n = 6) and 7.43 % ( n = 6), respectively. The concentra-tion compound W for non-pregnant women in serum and urine were(0.23 + 0.13) nmol/L and(79.94 + 41.49) pmol/mmolcreatinine,and there was a significant correlation between them (P ＜ 0.05). Conclusion: Radioimmunoassay for compoundW in human serum and urine is a sensitive,stabile method,and may be used in screening fetal congenital hypothyroidism.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 146-148 )