Construction of the Eukaryotic Expression Vector for hBMP-gene
Abstract：Objective:To construct the recombinant bacmid DNA of human bone morpbogenetic protein-2 (hBMP-2) gene and ex
press hBMP-2 by transfecting the insect cell line (Sf 9). Methods:The full-length coding region (1188 bp) for hBMP-2 in
cluding signal peptide,inter pro-peptide and mature peptide was inserted to the multi-cloning sites of baculovirus vector
pFastBacl which was controlled by the promoter pPolh, the recombinant plasmid (pFBBMP) was constructed, and pF-
BBMP was transformed into E. coli DH 10 Bac. After transposition, the positive colonies were selected and the bacmid DNA
was extracted. Confirmed by PCR,Sf 9 was transfected with recombinant bacmid DNA by liposome-mediated gene transfer
method. Results: The recombinant bacmid DNA which can directly transfect Sf 9 was produced,and the expression produc
tion (hBMP-2) was of 35.6% of total insect cell protein. Conclusion: The recombinant bacmid DNA is successfully con
structed by baculovirus vector,and hBMP-2 highly expresses in insect cells.
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Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 88-90 )