The Relationship between p53 and Apoptosis in Different Stages of Malignant Transformation of Large lntestine Epithelia
Abstract：Objective:To investigate the relationship between p53 and apoptosis in different stages of malignant transformation oflarge intestine epithelia. Methods: Using DNA nick end labeling technique for apoptotic cells, immunohistochemical stainingfor p53 protein and double staining for both, the density and distribution of the apoptotic cells and positive expression of p53protein were observed in situ in 21 cases of malignantly transformed adenomas and 27 cases of adenocarcinomas of the largeintestine. Fifteen cases of non-tumor mucosas were used as controls. Results: The density of the apoptotic cells in benign ar-eas of adenomas was significantly higher than that in non-tumor mucosas, malignant areas of adenomas or adenocarcinomasrespectively(all P ＜0.01). The densities of p53 protein positive cells in malignant areas of adenomas and adenocarcinomaswere significantly higher than those in benign areas of adenomas and non-tumor mucosas respectively( all P＜0.01). Thedistributions of p53 protein and apoptotic cells observed through double staining showed a tendency to decrease of apoptosisin p53 protein positive areas, while showing a tendency to increase in p53 negative areas. Conclusion:Probably through de-creasing in apoptosis, p53 mutations play a critical part in the formation and progression of carcinomas of large intestine.
Keywords：intestine neoplasms adenocarcinoma apoptosis protein p53
Publication Date：2000-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 665-667 )