Study on the Anti-cervical Cancer Activity and Mechanism of Active Components of Cordyceps Militaris Based on Network Pharmacology
DOU Yao
WANG Hongying
SUN Cong
ZHOU Xiaojing
GUO Yanxia
HOU Lu
WANG Weifang
Abstract：Objective To explore the molecular mechanisms of Cordycepin and cordycepic acid from Cordyceps militaris against cervical cancer(CC)from the perspective of systems biology,focusing on the multiple signal integration mechanisms.Methods Cordycepin and cordycepic acid were selected as the research objects.Based on the network pharmacology approach,the protein-protein interaction networks of their anti-CC effects were constructed.Using the CC Hela cell line,after determining the appropriate drug treatment concentrations,the inhibitory effects on cell proliferation and the induction of apoptosis were evaluated.The expression levels of key signaling pathway nodes in the network were analyzed.Results A total of 80 common targets between Cordycepin and CC and 131 common targets between cordycepic acid and CC were collected.After constructing the corresponding interaction networks,pathway enrichment analysis showed that Cordycepin-related targets were mapped to 73 pathways,and cordycepic acid-related targets were mapped to 166 signaling pathways.Cell experiments indicated that both could inhibit CC cell proliferation and promote apoptosis in a concentration-dependent manner,with cordycepic acid showing a stronger inhibitory effect on proliferation than that of Cordycepin.Further analysis of the gene expression of 9 key node proteins suggested that there were differences in the regulatory mechanisms between cordycepic acid and Cordycepin.Conclusion Cordycepin and cordycepic acid from Cordyceps militaris can play significant roles in the anti-CC mechanism by interfering with multiple signal transduction pathways and regulating the expression of multiple key target proteins.This study provides theoretical support and experimental evidence for systematically explaining the molecular mechanism of Cordyceps militaris against CC.
Keywords：Cervical cancerCordyceps militarisNetwork pharmacologyProtein-protein interaction networkSignal pathway
Publication Date：2025-09-15
Online Publishing Date：2025-12-22(First online date of this platform, not the publication date of the document)
Pages：7( 1-5,后插1-后插2,封3 )