Changes in Count,Volume,Functional and Activity of Pathogenic Inactivated Platelets During Storage

ZHU Lishuang; ZHANG Yuandan; WANG Hao

doi:10.16458/j.cnki.1007-0893.2025.10.005

Abstract：Objective To explore the effects of pathogen inactivation technology(PITs)on the quantity,morphology and function of apheresis platelets(PLT)during storage,and to provide a certain theoretical basis for pathogen inactivation of PLT.Methods Two therapeutic doses of machine-collected PLT from voluntary unpaid blood donors were randomly selected as the research subjects.One bag of PLT from each dose(two bags of PLT)was used as an experimental group,and the other bag was used as a control group.The PLT in the control group was not subjected to pathogen inactivation treatment,while the PLT in the experimental group was subjected to pathogen inactivation treatment.The aggregation function,activity changes,PLT count,PLT distribution width(PDW),and average PLT volume(MPV)of the PLT in the two groups were detected on the 0,2,6,and 8 days of PLT storage,respectively.The experiments were repeated three times.Results On the 2nd,6th,and 8th days,there was no statistically significant difference in PLT count,PDW,and MPV between the two groups compared with those on the 0th day(P>0.05).On the 6th and 8th days,the expression of PLT membrane glycoprotein α2β1 integrin(GPIa/Ⅱa)in the control group was lower than that on the 0th day.On the 2nd,6th,and 8th days,it was lower in the experimental group than that on the 0th day.On the 8th day,the expression in the experimental group was higher than that in the control group.The differences were all statistically significant(P<0.05).On the 2nd,6th,and 8th days,the expression of PLT membrane glycoprotein Ⅵ(GP6)in both groups was lower than that on the 0th day.On the 6th day,the expression in the experimental group was lower than that in the control group,and the differences were statistically significant(P<0.05).On the 2nd,6th and 8th days,the expression rate of PLT p-selectin(CD62p)in both groups was higher than that on the 0th day,and the re-expression rate of CD62p was lower than that on the 0th day.On the 6th day,the expression rate of CD62p in the experimental group was higher than that in the control group.On the 2nd and 8th days,the re-expression rate of CD62p in the experimental group was higher than that in the control group.On the 6th day,the re-expression rate of CD62p in the experimental group was lower than that in the control group.The differences were all statistically significant(P<0.05).Conclusion PITs have little influence on the quantity and form of mechanical PLT during storage,but they do have a certain impact on the aggregation function of PLT during storage.It may also lead to poor adhesion and aggregation functions of pathogen-inactivated PLTS with a storage period of 6 to 8 days after infusion.From this perspective,although PITs can reduce the incidence of pathogen infection during PLT storage,it will also affect the activity and function of PLT long-term storage to a certain extent.Therefore,how to balance the quality of PLT infusion and avoid pathogen infection of PLT will be a new challenge.