Exploring the Effect of Purple Sandalwood on Colon Cancer Cell line HCT116 Based on Endoplasmic Reticulum Stress PERK-ATF4-CHOP Signaling Pathway

WANG Yaxin; ZHENG Xiaoyue; FU Wenliang

doi:10.16458/j.cnki.1007-0893.2025.10.002

Abstract：Objective Based on the protein kinase R-like endoplasmic reticulum kinase(PERK)-activated transcription factor 4(ATF4)-transcription factor C/EBP homologous protein(CHOP)signaling pathway,this study aims to investigate the inhibitory effect of purple sandalwood on colon cancer cell proliferation and migration,induce cell apoptosis,and exert its anti colon cancer effect.Methods The effects of pterostilbene(PTE)on the proliferation,migration,apoptosis and cell cycle of HCT116 cells were observed by means of cell counting kit-8(CCK-8)assay,cell scratch assay,flow cytometry and other methods.The levels of intracellular reactive oxygen species(ROS)were detected using ROS probes,and HCT116 cells were divided into control group,PTE group,and PTE+GSK2606414(specific PERK pathway inhibitor)group.Western blot was used to detect the expression of PERK-ATF4-CHOP signaling pathway related proteins PERK,p-PERK,ATF4,CHOP,and apoptosis related proteins BAX and BCL-2.Results PTE inhibited the proliferation of HCT116 cells in a concentration dependent manner,with a half maximal inhibitory concentration(IC50)of 114.8 μmol·L-1;PTE can significantly inhibit the proliferation and migration of HCT116 cells,increase the early apoptosis rate of cells,induce cell cycle arrest in G1 phase of HCT116 cells,and induce excessive increase in ROS levels in HCT116 cells;The expression of pro apoptotic protein BAX increased and the expression of anti apoptotic protein BCL-2 decreased in HCT116 cells;The expression of PERK-ATF4-CHOP signaling pathway related proteins increased;Adding GSK2606414 can reverse the pro apoptotic effect of PTE on HCT116 cells.Conclusion PTE can significantly inhibit the proliferation,migration,and induce cell cycle arrest and apoptosis of colon cancer cell line HCT116;The induction of apoptosis in colon cancer cell line HCT116 by PTE may be related to the activation of the PERK-ATF4-CHOP signaling pathway.