Study on the Effect of Novel Coronavirus Infection on the Recovery of Lung Injury After Pulmonary Embolism in Rats
DENG Yicai
LIU Wenzhen
DONG Fang
TANG Shuzhi
CHEN Zhuo
Abstract：Objective To investigate the effects of novel coronavirus on the pathological changes of lung tissue,angiotensin-converting enzyme 2(ACE2)and angiotensin II(Ang Ⅱ)expression in rats with pulmonary embolism(PE).Methods Sixty rats were randomLy divided into four groups:blank control group(group C),PE model group(group M),novel coronavirus S protein group(group S)and PE+novel coronavirus S protein group(group MS),with 15 rats in each group.An acute PE model was established using gelatin sponge,followed by intraperitoneal injection of novel coronavirus S protein or an equivalent 0.9%sodium chloride injection.Lung tissue was collected at 1,5 and 10 days after injection for pulmonary examination to assess the degree of lung injury.Real-time fluorescence quantitative polymerase chain reaction(RT-QPCR)was used to detect the expression levels of ACE2 messenger RNA(mRNA)and Ang Ⅱ mRNA.The serum ACE2 content and lung ACE2 and Ang Ⅱ protein expression levels were detected by enzyme-linked immunosorbination(ELISA).Results The lung injury symptoms of rats in group M and group S were gradually relieved with time,and the serum ACE2 level,the expression of lung ACE2 mrna and ACE2 protein showed a downward trend,and gradually returned to normal level.In the MS group,the symptoms of lung injury continued to worsen,the serum ACE2 level remained high for a long time,and the expression of Ang Ⅱ mRNA and Ang Ⅱ protein in lung tissue was significantly increased.Conclusion The long-term high level of serum ACE2 and lung Ang Ⅱ protein expression after PE is an important mechanism of severe lung tissue injury caused by novel coronavirus.
Keywords：Novel coronavirusPulmonary embolismAngiotensin-converting enzyme 2Angiotensin IILung injury
Publication Date：2025-01-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 17-20,后插2-后插3 )