Study on the Protective Effect of Kangda Xin Oral Liquid against Doxorubicin-induced Cardiotoxicity in Zebrafish
QIAO Jianfeng
LIN Chao
XIONG Shangquan
LI Cuiyun
WANG Ting
ZENG Yajing
HE Shunyong
Abstract：Objective To evaluate the protective effect of Kangda Xin oral liquid on doxorubicin-induced cardiotoxicity in zebrafish and to explore its potential protective mechanisms.Methods The experiment was divided into blank control group,doxorubicin model group,Kangda Xin low-concentration group,Kangda Xin medium-concentration group,and Kangda Xin high-concentration group.Doxorubicin and Kangda Xin concentration gradients were designed,with 35 μg·mL-1 doxorubicin as the inducing concentration,and 5.9325×10-2 μg·mL-1,11.875×10-2 μg·mL-1,and 23.75×10-2 μg·mL-1 Kangda Xin as the low,medium,and high therapeutic concentrations,respectively.The rates of hatching and mortality in zebrafish were statistically analyzed at regular intervals.The malformations and heart rates of zebrafish were observed under a microscope.The cardiac cell apoptosis was observed by TUNEL fluorescence.JC-1 treatment was used to evaluate mitochondrial membrane potential(MMP).Oxidative damage was analyzed by detecting reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px).Results Compared with the blank control group,the heart rates of zebrafish in the doxorubicin model group and the Kangda Xin low-concentration group significantly decreased,while the heart rates in the Kangda Xin medium and high-concentration groups significantly increased,with statistically significant differences(P<0.05).Fluorescence staining showed that compared with the blank control group,the apoptosis of cardiac cells and mitochondrial damage were increased in the doxorubicin induced model group,and the co-treatment with Kangda Xin oral liquid effectively alleviated cell apoptosis and mitochondrial damage.Conclusion Kangda Xin oral liquid has a protective effect against doxorubicin-induced cardiotoxicity in zebrafish.
Keywords：Kangda Xin oral liquidDoxorubicinCardiotoxicityCardiac protectionZebrafish
Publication Date：2024-09-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1-5,后插1 )