Preliminary Study on the Function of Dihydrolipoamide-S-Succinyltransferase in HER2-Positive Breast Cancer
LING Sihui
PENG Zhiqiang
TONG Jianmeng
TAN Yawen
XIE Ping
Abstract：Objective To study the expression of dihydrolipoamide-S-succinyltransferase(DLST)in human epidermal growth factor receptor 2(HER2)-positive breast cancer patients and its impact on survival prognosis,as well as to clarify its possible involvement in the occurrence and development of HER2-positive breast cancer.Methods(1)Firstly,the CancerSEA database was used to screen for abnormal expression of DLST in various types of cancer,and then the Kaplan-Meier Plotter database was used to analyze the impact of DLST on the prognosis of various types breast cancer patients.(2)The CancerSEA database was used to explore and verify DLST-related signaling pathways.The interaction between DLST and proteins in breast cancer was studied through immunoprecipitation and GST-pull-down experiments.(3)The association between DLST and immune cell infiltration was analyzed using the TIMER 2.0 database.Results DLST was expressed at higher levels in HER2-positive subtype breast cancer patients,and the higher the expression level,the poorer the prognosis and the shorter the survival period of HER2-positive breast cancer patients.In invasive breast cancer,DLST was significantly negatively correlated with the apoptosis pathway.DLST was elevated in M2 macrophages in HER2-positive breast cancer.Neddylation-modified PTEN protein interacted with DLST.Conclusion DLST is highly expressed in breast cancer patient samples,and is positively correlated with the malignant progression of HER2-positive breast cancer,and is not conducive to the overall survival of HER2-positive breast cancer patients.Preliminary mechanism research suggests that DLST interacts with neddylation-modified PTEN protein,and inhibits tumor cell apoptosis and promotes M2 macrophage infiltration.Therefore,this study believes that DLST can be a potential therapeutic target and prognostic marker for clinical HER2-positive breast cancer patients.
Keywords：Human epidermal growth factor receptor 2-positive breast cancerDihydrolipoamide-S-succinyltransferaseApoptosisImmune infiltration
Publication Date：2023-09-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 6-10,后插2-后插3 )