Biofilm Formation, Molecular Typing and Drug Susceptibility Characteristics of Carbapenem-Resistant Klebsiella Pneumoniae
TU Hao-peng
CHEN Jun-wen
ZHANG Bo
XU Zhi-chao
ZHONG Wan-ying
CHEN Xian-di
CHEN Chong
MA Xiao-yu
BAI Bing
DENG Xiang-bin
PAN Wei-guang
XU Guang-jian
YU Zhi-jian
DENG Qi-wen
Li duo-yun
Abstract：Objective To explore the relationship between the biofilm formation characteristics and the multilocus sequence typing (MLST) and susceptibility of Klebsiella pneumoniae. Methods Klebsiella pneumoniae was collected from 2011-2016 in our hospital.The phenotypes of conventional phytotoxicity were detected by BD company's Phoenix TM 100 automatic bacterial susceptibility tester.The MLST typing was analyzed by PCR and 96-well crystal violet staining was used. Results Among the 36 strains, 24 biofilms were detected positive, and 12 biofilms were negative, 19 of which were weakly positive, 5 were positive, and no strong positive strain was found. The distribution of MLST is not concentrated. The number of MLST is 37, 11, 15, and 273. The Chinese name is the largest number of No type (NT), and ST37 and 273 are concentrated in the biofilm weak positive. The biofilm-positive amikacin, aztreonam, ampicillin, ciprofloxacin, ceftazidime, cefotaxime and levofloxacin resistance rates (including intermediate drug sensitivity) were 8.3％, 58.3％, 95.8 ％, 45.8 ％, 58.3 ％, 75.0％, and 50.0％. Conclusion The biofi lm formation ability of carbapenem Klebsiella pneumoniae is relatively strong and its distribution is not concentrated in MLST.
Keywords：Carbapenem resistanceKlebsiella pneumoniaeBiofilmMultilocus sequence typingCrystal violet staining
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )