Study on Warburg Effect in Bladder Tumor Cells and its Mechanism by Using Microlfuidic Chip Technology
GUO Wen-peng
RONG Zhi-bin
LI Ying-hong
CAI Zhi-ming
Abstract：Objective To study Warburg effect in bladder tumor cells and its molecular mechanism.Methods T24 bladder tumor cells were conventionally cultured by using RPMI-1460 medium. Microlfuidic chip platform was used to mimic the bladder tumor cells’ microenvironment and assay the metabolites of aerobic glycolysis.The content of nicotinamide-adenine dinucleotide phosphate (NADPH) was detected by spectrophotometric method.ResultsThe microfluidic chip was designed and fabricated in order to assay the metabolites. The descending trend of NADPH content was observed within a certain time extent which suggested that NADPH was consumed in a reduced form, and the generating source by the pentose phosphate pathway was inhibited.ConclusionTumor cells mainly participate in the aerobic glycolysis pathway and partly the pentose phosphate pathway.
Keywords：Microlfuidic chipBladder cancer cellsAerobic glycolysisMicroenvironment
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 1-3 )