Effect of resveratrol mediated by the PINK1/Parkin pathway on fibroblast-like synovial cells of osteoarthri-tis treated with H2O2
ZHANG Lixia
FAN Xuefei
CHEN Suhuan
ZHANG Mengyan
SHAO Yubao
ZHOU Jian
CHEN Xiaoyu
Abstract：Objective To investigate the effect of resveratrol(Res)on fibroblast-like synovial cells of osteoarthritis treated with H2O2,and to clarify the regulatory mechanism relationship of the PINK1/Parkin mitochon-drial autophagy signaling pathway in the above effects.Methods Fibroblast-like synovial cells(FLSs)were extracted from the synovial membranes of patients undergoing osteoarthritis surgery and divided into the control group and the experimental group.The experimental group was treated with H2O2(5 µmol/L)and then treated with different concentrations of Res(0,10,20,40,60,80,100 µmol/L).Cell viability assay(CCK-8)was used to detect the inhibition rate of cell proliferation.Cell reactive oxygen species were detected by DCFH-DA fluorescent probe.Mitochondrial reactive oxygen species were detected by MitoSOX Red fluorescent probe.Cell cycle was detected by flow cytometry.The expressions of proteins such as NLRP3,TNF-α,IL-1β,SOD2,Bax,Bcl-2,BNIP3,LC3B,p62,PINK1 and Parkin were detected by Western blot and immunofluorescence experiments.The role of the PINK1/Parkin mitochondrial autophagy signaling pathway in Res'inhibition of abnormal proliferation,inflammation and oxidative stress in FLSs was further verified using PINK1 and Parkin interfering RNA.Results Res signifi-cantly inhibited the enhancement of FLSs activity induced by H2O2,and reduced the abnormal proliferation,inflammation and oxidative stress levels of FLSs;Res inhibited the expressions of NLRP3,TNF-α,Bcl-2 and p62 proteins,while promoting the expressions of SOD2,Bax,BNIP3,LC3B,PINK1 and Parkin proteins.H2O2 inhib-ited the PINK1/Parkin signaling pathway,while Res activated the PINK1/Parkin pathway.The use of PINK1 and Parkin interfering RNA exacerbated cellular inflammation and oxidative stress.Conclusion Res activates the PINK1/Parkin mitochondrial autophagy signaling pathway by increasing the expression of PINK1 and Parkin proteins,alleviating abnormal proliferation,inflammation and oxidative stress in fibroblast-like synovial cells of osteoarthritis induced by H2O2.
Keywords：resveratrolosteoarthritisfibroblast-like synovial cellsPINK1/Parkin signaling path-wayinflammationoxidative stress
Publication Date：2026-02-10
Online Publishing Date：2026-02-07(First online date of this platform, not the publication date of the document)
Pages：8( 363-370 )