Exploration of the application value of urinary ACE2 and PAX2 in early kidney injury of congenital kid-ney and urinary tract malformations
YU Pao
ZHU Feng
WANG Zi
ZHANG Yin
GE Zheng
ZHOU Bi
Abstract：Objective To investigate the expression levels of urinary ACE2 and PAX2 proteins in children with congenital anomalies of the kidney and urinary tract(CAKUT)and their association with acute kidney injury(AKI),and to assess the diagnostic value of these proteins as biomarkers for early renal injury in CAKUT.Methods A total of 266 children aged 1～18 years with CAKUT were enrolled.They were divided into the CAKUT with AKI group(n=68)and the CAKUT without AKI group(n=198)in accordance with the Kidney Disease:Improving Global Outcomes(KDIGO)criteria,along with a matched healthy control group(n=45).The levels of angiotensin-converting enzyme 2(ACE2)and paired box 2(PAX2)in morning urine were measured by means of enzyme-linked immunosorbent assay(ELISA).Statistical analysis was carried out in combination with renal function indica-tors such as serum creatinine and estimated glomerular filtration rate(eGFR).The diagnostic efficacy was assessed via receiver operating characteristic(ROC)curve analysis,and the correlation between the markers and eGFR was investigated using Pearson correlation analysis.Results The levels of urinary ACE2 and PAX2 in the CAKUT-AKI group were significantly higher compared to those in the non-AKI group and the healthy group,and they exhibited a stepwise increase as the AKI stages progressed(P<0.01).There was no statistically significant difference between the non-AKI group and the healthy group(P>0.05).Both ACE2 and PAX2 demonstrated strong negative correlations with eGFR(r=-0.72,P<0.01;r=-0.805,P<0.01).The area under the curve(AUC)for ACE2 alone in diagnosis was 0.917(cut-off value 177.8 ng/mg Cr,sensitivity 80.9%,specificity 86.4%);the AUC for PAX2 alone in diagnosis was 0.853(cut-off value 11.1 pg/mg Cr,sensitivity 80.3%,specificity 76.1%);the com-bined testing AUC increased to 0.956(sensitivity 88.2%,specificity 87.9%),which was significantly superior to single indicators(P<0.01).Conclusion Urinary ACE2 and PAX2 can be utilized to aid in the diagnosis of CAKUT complicated by acute kidney injury,demonstrating favorable diagnostic efficacy.
Keywords：congenital anomalies of the kidney and urinary tractangiotensin-converting enzyme 2paired box gene 2acute kidney injurybiomarkers
Publication Date：2026-01-10
Online Publishing Date：2026-01-19(First online date of this platform, not the publication date of the document)
Pages：7( 139-145 )