The inhibitory effect and molecular mechanism of 6-gingerol on human multiple myeloma cells
KONG Chunfang
LI Anna
KE Bo
DING Weirong
LIU Tingting
FU Huan
ZHANG Tingting
JIN Chenghao
WU Mei
Abstract：Objective To investigate the inhibitory effect and elucidate the molecular mechanism of 6-gingerol on human multiple myeloma cells.Methods The human multiple myeloma cell lines RPMI 8226 and ARH77 were cultured in vitro,followed by treatment with varying concentrations(50,100,200,300,400 μmol/L)of 6-gingerol.The inhibitory effect on cell proliferation was assessed using the CCK-8 assay.Flow cytometry was employed to evaluate cell apoptosis and cycle distribution.Additionally,qRT-PCR and Western blotting techniques were utilized to analyze gene and protein expression levels.Results The proliferation of RPMI 8226 and ARH77 cells was dose-and time-dependently inhibited by 6-gingerol,leading to the induction of apoptosis with statistically significant differences(P＜0.05).Further mechanistic investigations revealed that treatment with 6-gingerol arrested RPMI 8226 cells in the G0/G1 phase,resulting in a significant increase in Bax levels and a decrease in Bcl-2 mRNA and c-Myc mRNA levels(P＜0.05).Additionally,it significantly upregulated the expression of Bax,Cleaved-PARP,Cleaved-caspase3,P53,and p-AKT proteins while down regulating the expression of Bcl-2 protein(P＜0.05).Conclusions The compound 6-Gingerol exhibits inhibitory effects on the proliferation and induction of apoptosis in MM cells,as well as cell cycle arrest at the G0/G1 phase.Its mechanism of action is likely associated with the suppression of the AKT signaling pathway,downregulation of Bcl-2 family protein expression,and inhibition of c-Myc expression.
Keywords：multiple myeloma6-gingerolcell proliferationcell apoptosissignal pathway
Publication Date：2024-12-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 3291-3297 )