Liraglutide ameliorates high glucose-induced endothelial cell injuryvia Nrf2
SUN Yunlong
MENG Zhe
WANG Xijia
GAO Lu
Abstract：Objective This study aimed to investigate the effect and mechanism of Liraglutide on high glucose-induced vascular endothelial cell injury.Methods Human umbilical vein endothelial cells(HUVECs)were cultured and divided into 4 groups:control,high glucose stimulation(HG),Liraglutide,and Liraglutide+HG group.ELISA was used to detect the secretion of inflammatory cytokines in each group,kits the levels of intracellu-lar reactive oxygen species(ROS),the activity of SOD2 and Gpx4,and the level of MDA,and Western blot and immun of luorescence method nuclear translocation of Nrf2.Results The HG groupexhibited a significantly higher levels of tumor necrosis factor(TNF-α),interleukin(IL-1),IL-6,as well as the levels of intracellular ROS and MDA,but less activity of SOD2 and Gpx4,less contents of NO,and less nuclear translocation of Nrf2,as com-pared with the control group.Compared with the HG group,the Liraglutide+HG grouppresented significantly higher levels of TNF-α,IL-1,and IL-6,but lower levels of intracellular ROS and MDA,less activities of SOD2 and Gpx4,less contents of NO and less nuclear translocation of Nrf2.Conclusion Liraglutide suppresses high glucose-induced endothelial cell injury by increasing the nuclear translocation of Nrf2.
Keywords：endothelial cellshyperglycemialiraglutideoxidative stressNF-E2-related factor 2
Publication Date：2024-08-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 2051-2055 )