Application of leukocyte-and platelet-rich fibrin in dental pulp repair of rat

WANG Ting; HE Li

doi:10.3969/j.issn.1006-5725.2024.14.007

Abstract：Objective To explore the effect of leukocyte-and platelet-rich fibrin(L-PRF)as a capping agent in the modulation of inflammation in rat dental pulp.Methods The rat model of pulpitis was established by lipopolysaccharide(LPS).A total of 30 Sprague-Dawley rats were randomly divided into five groups:Normal control group,Model group,iRoot BP PLUS group,L-PRF group,iRoot BP PLUS+L-PRF group.Samples were collected on the 14th day after pulp capping.Micro-CT scans,HE and Masson staining,qRT-PCR assays were performed to observe pulp tissue inflammatory and hard tissue formation.Results Micro-CT results:no hard tissue formation was seen in the LPS group,plaque calculus was seen in the L-PRF group,and a more complete calcified bridge was seen at the perforation of the pulp in the BP and combined groups;HE and Masson staining results:the LPS group had the most severe inflammation and no hard tissue formation;the combined group had the least inflamma-tion and the most complete and continuous hard tissue formation,and there was a statistical difference between the two groups(P<0.01);There was no statistical difference in the degree of inflammatory reaction and hard tissue formation between the BP group and the combined group(P>0.05);qRT-PCR results:the relative expression levels of TNF-α,IL-1β,and IL-6 mRNA in the pulp tissues of all experimental groups were reduced compared with those of the LPS group,but the differences between the L-PRF and LPS groups were not statistically signifi-cant(P>0.05).Conclusion The combination of L-PRF and iRoot BP PLUS as a composite pulp capping material can be used for the repair of dental pulp tissue and the regulation of inflammation in dental pulpitis in rats.