Effects of allergens on the expressions of IL-18,IL-18BPa and IL-18Rα in blood CD4+Th1 cells of patients with allergic rhinitis
WANG Junling
ZHAN Mengmeng
GU Fangqiu
LI Yifei
ZHANG Zhaolong
ZHAO Congyi
ZHAO Danyang
ZHENG Hui
ZHANG Yijie
QIN Bingyu
Abstract：Objective To investigate the effects of allergens on the expressions of IL-18,IL-18BPa and IL-18Rα protein in peripheral blood CD4+Th1 cells of healthy control subjects(HC)and patients with allergic rhi-nitis(AR),and on the expressions of IL-18,IL-18BPa and IL-18Rα mRNA in the peripheral blood CD4+T cells.Methods Blood samples were collected from patients with rhinitis for negative skin prick test(AR-),rhinitis for positive skin prick test(AR+)and HC.Flow cytometry was used to evaluate the effects of allergens on the expres-sions of IL-18,IL-18BPa and IL-18Rα protein in CD4+Th1 cells.The expressions of IL-18,IL-18BPa and IL-18Rα mRNA in CD4+T cells were determined by qPCR.Results Compared with HC,increased IL-18 while de-creased IL-18BPa expressions in Th1 cells of AR-and AR+patients were observed,increased IL-18Rα expression in Th1 cells of AR+patients was also found.Additionally,allergens induced elevated expression of IL-18Rα pro-tein in Th1 cells of HC,and induced elevated mRNA expressions of IL-18,IL-18BPa and IL-18Rα in isolated blood CD4+T cells of AR+patients and HC.Conclusion Allergens may be involved in the pathogenesis of AR by inducing the expressions of IL-18 and IL-18Rα in blood CD4+Th1 cells.
Keywords：allergic rhinitisCD4+Th1 cellIL-18IL-18Rα
Publication Date：2024-06-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1513-1518 )