Proanthocyanidins promotes neurite outgrowth of dorsal root ganglion neurons in rat
CHEN Yingxiu
GUO Yingqi
ZHANG Huimei
ZHANG Qi
WEN Jinkun
LUO Li
LI Lixia
Abstract：Objective To investigate the effect of proanthocyanidins(PC)on the neurite outgrowth of rat dorsal root ganglion(DRG)neurons.Methods In vitro,primary rat DRG neurons were cultured wtih a series of concenteation of PC to assess the effect of PC on the number and length of neurites as well as the morphology of growth cone.In vivo,the expression of growth associated protein 43(GAP43)in the early stage of injury was detected using the sciatic nerve crush model.Finally,the impact of PC on nerve growth factor(NGF)expression in DRG neurons was evaluated in vitro using immunofluorescence and ELISA.Results PC significantly increased the number and length of neurites and the number of pseudopodium in growth cones of DRG neurons.PC also promoted the expres-sion of GAP43 in the early stage of sciatic nerve injury in rats and enhanced the expression of NGF in DRG neurons.Conclusion PC may promote the neurite outgrowth by increasing the expression of NGF in DRG neurons.
Keywords：proanthocyanidinsneuronsneurite outgrowthnerve growth factor
Publication Date：2024-05-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 1357-1363 )