Therapeutic effect of edaravone combined with rapamycin on neural injury in rats with subarachnoid hem-orrhage

AN Chaowang; WANG Zheng; LI Jianmin

doi:10.3969/j.issn.1006-5725.2017.21.012

Abstract：Objective To investigate the therapeutic effect of edaravone(Ed)combined with rapamycin (RAP)on neural injury in rats with subarachnoid hemorrhage(SAH),and to provide the evidence for clinical medication. Methods 100 male Sprague-Dawley rats were randomly divided into the following 5 groups:sham operated(Sham)group,SAH group,RAP group,Ed group and RAP+Ed group. A rat model of SAH was estab-lished by using the 2 injection of the classic pillow.Learning and memory function assessment was performed by us-ing Morris water maze. The morphological changes of neurons in hippocampus were observed by using optical mi-croscopy. The MDA content was measured by the thiobarbituric acid method. The expressions of autophagy marker proteins(Beclin-1 and LC3-Ⅱ)were detected by immunohistochemistry assay.Results The number of dead neu-rons,MDA content,the expressions of Beclin-1 and LC3-Ⅱwere higher in SAH group than those in Sham group, but learning and memory function index was worse than that in Sham group(P<0.05).The number of dead neu-rons,MDA content in RAP or Ed group were lower than those in SAH group(P < 0.05,respectively),while the expressions of Beclin-1 and LC3-Ⅱwere higher and learning and memory function index was better in RAP or Ed group than those in SAH group(P<0.05,respectively).The number of dead neurons,MDA content in RAP+Ed group were lower than those in RAP or Ed group(P < 0.05,respectively),but the expressions of Beclin-1 and LC3-Ⅱwere higher and learning and memory function index was better in RAP+Ed group than those in RAP or Ed group(P < 0.05,respectively). Conclusions Edaravone combined with RAP had better therapeutic effect on neural injury in rats with SAH,which was associated with enhancing the autophagy of neural cells.