The experimental research of microRNA-96-5p downregulating mTOR expression of BGC-823 cells
YU Zhaoyan
DING Jie
YUAN Ping
ZHANG Zhongmin
Abstract：Objective To investigate the effect of microRNA-96-5p(miR-96-5p)on expression of mam-malian target of rapamycin(mTOR)in gastric cancer cells. Methods Lentiviral vector,LV-hsa-mir-96-5p was transfected into BGC-823 gastric cancer cells. The expression of miR-96-5p and mTOR mRNA after transfection was detected by qRT-PCR,and the expression of mTOR protein was detected by Western blot. Results At 72 h after transfection,the expression level of miR-96-5p in the experimental group was 20.4 times of that in the blank control group(P<0.05),while the expression level of mTOR gene was downregulated to 0.47 times of that in the blank control group (P < 0.05),and mTOR protein relative expression was significantly reduced compared with the control group (P < 0.05). Conclusion Overexpression of miR-96-5p can downregulate the expression of mRNA and protein of mTOR gene in gastric cancer cells,thus,mTOR may be one of the downstream target genes of miR-96-5p.
Keywords：gastric cancermicroRNA-96-5pmammalian target of rapamycin
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2991-2994 )