Establishment of a rat model of heart and kidney transplantation

JIN Zhongda; JU Chunhua

doi:10.3969/j.issn.1006-5725.2017.16.008

Abstract：Objective To explore the surgical procedure for combined heart and kidney transplantation in rats. Methods Inbred Lewis rats were used as donors and recipients. The left kidney and the heart were removed from the donors,the donor kidney was therefore transplanted to the left side. Revascularization of renal artery was constructed end-in-end by modified sleeve anastomosis. Renal veins were anastomosed using stenting technique. Ureters were anastomosed using end-to-end interrupted suture technique. The aortic and pulmonary arteries were anastomosed to the recipient abdominal aorta and inferior vena cavaLewis rats receiving right nephrectomy were assigned to a control group. Results The time of warm ischemia was 10s,and the donor nephrectomy time was (30 ± 3.0)min;the removal of donor heart was(6 ± 1.8)min,the renal cold ischemia time was(40 ± 3.2)min and heart was(70 ± 4.1)min,the meantime for recipients operation was(95 ± 5.4)min,and the graft survival rate was 80.9%. Renal function and heart rate did not differ significantly between the two groups 30 days after the procedure. Conclusions The new model for simultaneous heart and kidney transplantation in rats is feasible and reliable. This technique can be applied to basic research on multiple organ transplantation immunity.