Effect of Yiqixue Recipe on Ovary Apoptosis and Expression of Bim in Ovarian Granulosa Cells of Con-trolled Ovarian Hyperstimulation Rats
YANG Zhen
GUO Xinyu
DENG Weimin
ZHANG Jinyu
YUAN Renfei
TANG Lulu
Abstract：Objective To assess the effects of Jinghou Zengzhi Recipe(JHZZR),a Chinese prescription with the action of tonifying Qi and blood ,on the ovary apoptosis and expression of Bim in ovarian granulosa cells (GCs) of controlled ovarian hyperstimulation(COH) rats , and analyze the possible therapeutic mechanism. Methods A model of COH rats were prepaered and 30 female SD rats were randomly divided into 5 groups , including control group,positive control group,low,medium and high concentration group in six rats in each group. The apoptosis index(AI)in ovarian GCs were detected by TUNEL ,and the expression of Bim by qPCR. Results The AI of ovarian GCs in high and medium concentration group were obviously lower(P < 0.05)than that in the positive control group,and there is no significant differences(P > 0.05)comparing with control group. The mRNA levels of Bim were lower(P<0.05)in all concentration group than those in positive control group ,but still higher(P<0.05)than that in control group. And there is no significant differences(P>0.05)during the three concentration groups in Bim mRNA. Conclusion JHZZR can inhibit the ovarian GCs apoptosis of COH rats through decreasing the expression of Bim mRNA ,which improve the quality of ovarian follicle.
Keywords：Yiqixue recipeJinghou Zengzhi RecipeControlled ovarian hyperstimulationgranulosa cells apoptosisBim
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1593-1596 )