Effects of exogenous hydrogen sulfide inhalation on brain edema induced by experimental stroke in rats ;and mechanism study
Abstract：Objective To investigate the effects of exogenous H2S gas on brain edema induced by experimental stroke and underlying mechanisms. Methods Rats were divided into 6 groups, shame, I/R, H2S40, H2S80, ICPBS and ICPKCi. Rats of H2S40 and H2S80 groups underwent 2 h MCAO and received 40 ppm or 80 ppm H2S inhalation for 3 h at the beginning of reperfusion. Rats of ICPBS and ICPKCi were injected with 5 μL PBS or PKC inhibitor Go6983 first and received 40 ppm for 3 h. The effects of H 2S were investigated by evaluating neurological function, infarct size, and brain edema volume. Western blot was used to measure AQP4 protein expression at 24 h after reperfusion. PKC inhibitor Go6983 was injected into intracerebral ventricular to explore the possible mechanisms for the neuroprotective effects of H2S. Results 40 and 80 ppm H2S inhalation significantly reduced neurological deficits, infarct size, and brain edema volume after MCAO (P < 0.05). The expression of AQP4 in the peri-infarct area of brain was also inhibited after inhalation of H2S(P < 0.05). The PKC inhibitor reversed the neuroprotection of H2S with an increased AQP4 expression at the same time (P < 0.05). Conclusion 40 or 80 ppm H2S inhalation attenuated brain edema , reduced infarct volume and improved neurologic function in a rat experimental stroke model. The therapeutic benefits of H2S inhalation might be caused by downregulation of AQP4 expression under possible regulation of PKC.
Keywords：Hydrogen sulfideMiddle cerebral artery occlusionBrain edemaAquaporin 4Protein kinase C
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2291-2294 )